목화 陸地棉과 亞細亞棉 種間雜種의 胚珠培養時 生長調節劑와 炭素源의 影響

• 영문명: Effects of growth regulators and carbon sources on ovule culture of interspecific hybrid between Gossypium hirsutum and Gossypium arboreum
• 발행기관: 한국육종학회
• 저자명: Kyung Soo Min(閔庚洙) Hong Jae Park(朴洪在)
• 간행물 정보: 『한국육종학회지』Vol.29 No.3, 263~269쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1997.09.30

영문 초록

This study was performed to increase the efficiency of interspecific hybrid production between Gossypium hirsutum and Gossypium arboreum via in vitro ovule culture. Immature ovules dissected from the interspecific crossed cotton fruit at 4 days after pollination were cultured in Shenck-Hilderbrandt(SH) liquid medium supplemented with various growth regulators and carbon sources. The best results were observed from the combination of 5mM IAA, 0.005mM GA, 0.05mM Kinetin with 18.5% of embryo survival and 10.6% plant regeneration. Glucose and sucrose were considered as reasonable carbon sources for the ovule culture of cotton achieving the highest frequency of plant regeneration at 150mM and/or 200mM, respectively.

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키워드

Gossypium hirsutum G. arborium Interspecific hybrid Ovule culture Growth regulator Carbon sources