In vitro culture of hybrid ovule between Fagopyrum esculentum Moench and F. homotropicum Ohnishi

• 발행기관: 한국육종학회
• 저자명: Sun Hee Woo(禹仙熙) Taiji Adachi(足立泰二) Seung Keun Jong(鄭丞根)
• 간행물 정보: 『한국육종학회지』Vol.29 No.3, 289~294쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1997.09.30

영문 초록

Interspecific hybridization in buckwheat is of great importance to transfer desirable genes from wild species to cultivars as well as to broaden genetic variation in cultivars. In vitro ovule culture following the bud pollination was made to overcome postzygotic cross/self-incompatibility in interspecific hybridization between Fagopyrum esculentum Moench (common heterostylous buckwheat) and F. homotropicum Ohnishi (homostylous buckwheat). The percentage of fertilized ovaries of F. esculentum flowers (thrum-type) pollinated with F. homotropicum was 50%, while that of the reciprocal cross was as low as 10% and their ovaries were smaller. Vigorous pollen tube elongation following the bud pollination indicated the possibility for overcoming of breeding barriers in interspecific crosses between buckwheat species. Pre-embryo formation was observed 48 hours after pollination and ovules cultured up to 5 days after pollination tended to develop callus. But ovules isolated 11 days after pollination had apparently lost their viability. Hybrid plantlets were produced from only the ovules dissected 5 days after bud pollination. Regeneration rate was better in ovule culture on White medium than other mediums tested. Interspecific hybrid plants between F. esculentum and F. homotropicum were regenerated with the rate of 24.2% in average.

목차

ABSTRACT

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

摘要

LITERATURES CITED

키워드

Common buckwheat(Fagopyrum esculentum Moench) F. homotropirnm Ohnishi Interspecific cross Pollen tube elongation Ovule culture