시호 배양소포자의 flow cytometric 특성

• 영문명: Flow cytometric characterization and sorting of cultured Bupleurum falcatum Microspores
• 발행기관: 한국육종학회
• 저자명: 박충헌(Chung Heon Park) 성낙술(Nak Sul Seang) 김관수(Kwan Su Kim) 이승택(Seung Tack Lee) 피터 포울(Peter Pauls) 대릭 슐츠(Derek Schulze)
• 간행물 정보: 『한국육종학회지』Vol.29 No.2, 238~243쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1997.06.30

영문 초록

Under the appropriate culture conditions microspores could be induced switch their development pathway from pollen to embryo formation. Many efforts are under way to elucidate the molecular, physiological and morphological changes associated with embryogenesis. A goal of our research program is to characterize those cells in Bupleurum falcatum microspore culture that are active. For this purpose we have used flow cytometric and epifluorescent techniques. The knowledge obtained from these types of studies will give us insight into early stage in plant development and may lead to the application of microsporederived from haploid plants for breeding in recalcitrant species. Activity of cultured microspore was different from the developmental stage that of the tetrad, uni-nucleate, bi-nucleate and matured pollen were 15.1, 36.4, 84.0 and 56.4%, respectively. Optimum culture medium was determined by the OM media as 79.4% of active microspore presence 9days after culture. Korea originated cultivar was more active than Japan’s cartivar “Misima” during the micorspore culture.

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키워드

Micorspore culture Bupleurum falcatum Flow cytometry