生長調節物質이 벼 葯培養에 미치는 影響

• 영문명: Effects of growth regulators on rice anther culture
• 발행기관: 한국육종학회
• 저자명: Je Hwan Kim(金濟桓) So Hyun Baek(白蘇鉉) Jeom Ho Ryu(柳點鎬)
• 간행물 정보: 『한국육종학회지』Vol.29 No.2, 200~209쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1997.06.30

영문 초록

This study was carried out to investigate the effects of growth regulators on the microspore surival rate, pollen dimorphism, microspore-originated calli formation and plant regeneration. The anthers of rice were cultured on the N₆ basal medium supplemented with the single or the combination of α-naphthalen acetic acid (NAA), 2, 4-dichlorophenoxy acetic acid(2, 4-D) and Kinetin. Microspore surival rate was not greatly different among growth regulators in 5 days of culture, but it was the highest on the medium with 1 mg/L NAA(81.9%), and the lowest on the medium with 4 mg/L Kinetin (40.3%) in 20 days of culture. Appearance rate of S pollen was low on the medium with 4 mg/L, Kinetin (24.2%), and high on the medium with 1 mg/L NAA (75.6%) Both of callus induction and plant regeneration were good on the medium supplement with NAA or 2, 4-D each, the combination of NAA with Kinetin, and 2, 4-D with Kinetin. The rate of induction was best on the medium with 1 mg/L 2, 4-D (55.1%). But the plant was not differentiated in calli induced from the medium with only Kinetin supplemented. The frequency of plant regeneration was excellent in calli induced from the medium with 2 mg/L NAA (13.5%)

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키워드

Rice Anther culture Growth regulator