Air-lift bioreactor에서 쪽 毛狀根의 생장과 indigoid 생산

• 영문명: Growth and indigoid production of polygonum tinctorium lour hairy root. in air-lift bioreactor
• 발행기관: 한국육종학회
• 저자명: Hee Kyung Chun(全禧경) Young Am Chae(蔡永岩)
• 간행물 정보: 『한국육종학회지』Vol.29 No.2, 223~228쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1997.06.30

영문 초록

This study was conducted to select the most effective condition for the commercial application of indigoid production and growth in hairy root of Polygonum tinctorium Lour. transformed by Agrobacterium rhizogenes strain 11325 and 15834 in the air-lift bioreactor. Growth and indigo of hairy roots was turn into the stationary phase after 20 days, which suggested that the subcultural period might be 30 days. Higher indigo and indirubin contents were detected 20 (400 g/g) and 30(215.6 g/g) days, respectively, after add of 200mg/1 indole as precursor at the beginning of culture in the air-lift bioreactor. However, only indigo was detected in the control. Pluronic F-68 was effective for growth and indigo production as elicitor at 0.0lg/l but over this level increased working volume which caused contamination the medium in air-lift bioreactor. Silicon-based antifoam C-emulsion treatment with 200ppm supress effectively foam overflowing.

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키워드

Polygonum tinctorium Lour. Hairy root Agrobacterium rhizogenes Indigo Indirubin Antifoam Indole Pluronic F-68 Bioreactor