芍藥의 小胞子 培養에 의한 식물체 재분화

• 영문명: Plant regeneration from microspore culture of Paeonia lactiflora Pall.
• 발행기관: 한국육종학회
• 저자명: 김난영(Nan Young Kim) 손재근(Jae Keun Sohn)
• 간행물 정보: 『한국육종학회지』Vol.28 No.4, 415~419쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1996.12.30

영문 초록

This study was carried out to determine the optimum conditions for the embryo formation in the shed microspore culture of Paeonia lactiflora Pall. The highest frequency of microspore division(38.7%) and embryo formation(123 embryos/100 anthers) was obtained at the shed microspore density of 3.1×10⁴ cells/㎖. Cold pretreatment(5℃, 10 days) of anthers enhanced the division of shed microspores and embryo formation. Pretreatment of anthers at low temperature resulted in the formation of more embryos from microspores. The concentration of 3% sucrose gave better results than that of the sucrose with sorbitol in the division of shed microspores and embryo formation. The preculture of anthers on solid medium was not effective for the embryo formation from shed microspore culture of Paeonia lactiflora.

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ABSTRACT

緖言

材料 및 方法

結果 및 考察

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引用文獻

키워드

Shed microspore Microspore culture Embryo formation Paeonia lactiflora