DNA-DNA in situ hybridization에 의한 옥수수 rRNA 遺傳子座의 決定

• 영문명: Localization of rRNA genes by DNA-DNA in situ hybridization in zea mays
• 발행기관: 한국육종학회
• 저자명: 윤성중(Song Joong Yun) 김제환(Je Hwan Kim)
• 간행물 정보: 『한국육종학회지』Vol.24 No.4, 363~367쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1992.12.30

영문 초록

A modified DNA-DNA in situ hybridization procedure was used to localize rRNA genes on chromosomes using cytological preparations. ³H-labeled 18S, 5.8S, and 26S maize rRNA genes specifically hybridized to the nucleolus organizor region(NOR) of pachytene and diplotene chromosme, indicating that 18S, 5.8S, and 26S rRNA genes were present in the NOR of maize chromosome 6. This also suggested that the in situ hybridization procedure used in this study could be applicable to localize other genes on chromosomes and investigate their structural characteristics using cytological preparations.

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ABSTRACT

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키워드

DNA-DNA in situ hybridization localization nucleolus organizor region rRNA genes Zea mays