Enhancement of chitinolytic activity of by co-expression of endochitinase and chitobiosidase genes

• 영문명: Endochitinase와 Chitobiosidase 유전자의 동시발현에 의한 키틴분해 활성의 증가
• 발행기관: 강원대학교 산업기술연구소
• 저자명: 김정태(Kim, Jungtae) 최신건(Choi,Shin-Geon)
• 간행물 정보: 『산업기술연구』vol.30, 69~74쪽, 전체 6쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2010.09.30

영문 초록

Chitinolytic activity was enhanced by coexpression of endo-chitinase gene(chiA) and chitobiosidase gene (chiB) from Serratia marcescens KFRI314 using constitutive expression vector, pHCEIA, in E. coli. Coexpression vector was constructed by inserting ribosome binding site (RBS)into junction between two chitinase genes. SDS-PAGE analyses showed that two chitinase were constitutively expressed while E. coliclones expressing two chitinases simultaneously increased halo size on colloidal chitin plate. Furthermore, the chitinolytic activities were much enhanced in coexpressed clones when degradation patterns of substrate analogues such as 4-MU-(NAG), 4-MU-(NAG) 2 ,4-MU-(NAG) 3 were used. Consequently, the combined use of endochitinase and chitobiosidase greatly increased overall chitinolytic activities on recombinant E. coli clones

목차

1. INTRODUCTION

2. MATERIALS AND METHODS

3. RESULTS AND DISCUSSION

키워드

Serratia marcescens chitinase 키틴분해 상승효과 Serratia marcescens chitinase chitindegradation synergistic effect