Cloning of Serratia marcescens KFRI314 chitinase genes and its role on chitin degradation

• 영문명: Serratia marcescens KFRI314 chitinase 유전자의 클로닝과 키틴분해에 관한 효소의 역할
• 발행기관: 강원대학교 산업기술연구소
• 저자명: 김정태(Kim, Jungtae) 최신건(Choi, Shin-Geon)
• 간행물 정보: 『산업기술연구』vol.30, 61~68쪽, 전체 8쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2010.09.30

영문 초록

Three chitinase genes (chiA,chiB,and chiC)were cloned into E.coliby PCR amplification from Serratia marcescens KFRI314.The sizes of cloned chitinase genes were1692 bp,1500 bp, and 1443 bp which correspond to 563,499, and 480 aminoacids, respectively. Recombinantchitinaseswereoverexpressedusing pHCEIA expression vector and purified to homogenity. The molecular weights of chitinases were about 60kDa, 50kDa, 52kDa, respectively. Optimum pHs were around pH 5~6 and optimum temperatures were 45∼50℃ while 90% of enzyme activities were stable up to 50℃. The specific activities of ChiA, ChiB, and ChiC were 233.1, 278.8,111.3 μ ㏖ (min) -1 mg –1 against colloidal chitin. From experiments using TLC and fluorescent substrate analogues, it was demonstrated that ChiA was endo-chitinase while ChiB and ChiC were chitobiosidase.

목차

1 .INTRODUCTION

2. MATERIALS AND METHODS

3. RESULTS AND DISCUSSION

키워드

Serratia marcescens Chitinase 유전자 키틴분해 Serratia marcescens Chitinase Gene Chitin degradation