Biochemical and Molecular Analysis of OsPAP1: A Phosphate Starvation Induced Purple Acid Phosphatase Gene from Rice

• 발행기관: 한국육종학회
• 저자명: Yeon Jae Hur Young Byung Yi Tae Ho Kim Doh Hoon Kim
• 간행물 정보: 『한국육종학회지』Vol.42 No.5, 455~462쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2010.10.30

영문 초록

Purple acid phosphatase is important for phosphorus remobilization in plants, but its role in plant adaptation to low phosphorus availability is not known. The cDNA encoding O. sativa purple acid phosphatase (OsPAP1) has 1008 bp with an open reading frame of 335 amino acid residues. The amino acid sequence of OsPAP1 cDNA shows of 50-51% identity with other plant purple acid phosphatases. OsPAP1 was expressed in rice plants and in cell cultures in the absence of phosphate (P i ). The expression was organ-specific with the strongest expression in P i -deprived roots. Functional expression of the OsPAP1 gene in the transgenic Arabidopsis line was confirmed by northern and western blot analysis. OsPAP1 overexpression lines had higher phosphatase activity than wild-type. Overexpression of OsPAP1 in Arabidopsis plants resulted in increased P i accumulation under P i sufficient condition. These results show that the OsPAP1 gene represents more efficient P i uptake and can be used to develop new transgenic dicotyledonous plants.

목차

INTRODUCTION

Materials and Methods

Results and Discussion

REFERENCES

키워드

Purple acid phosphatase Phosphate Transgenic Arabidopsis Rice