Changes in Polygalacturonase and Ethylene Biosynthesis of Three Varieties of Apple During Fruit Ripening

• 발행기관: 한국육종학회
• 저자명: Se Hee Kim Sang Eun Han Hye Eun Lee Mi-Ae Cho Il Sheob Shin Jeong-Hee Kim Kang-Hee Cho Dae-Hyun Kim Jeong Hwan Hwang
• 간행물 정보: 『한국육종학회지』Vol.42 No.5, 481~487쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2010.10.30

영문 초록

The ripening behavior of three apple cultivars, ‘Tsugaru’, ‘Hongro’ and ‘Fuji’ was distinctive and the involvement of POLYGALACTURONASE(PG) in the fruit softening process was confirmed to be ethylene dependent. Fruit softening is genetically coordinated by the action of several cell wall enzymes, including PG which depolymerizes cell wall pectin. Also, loss of firmness is associated with increasing of the ripening hormone, ethylene. In this work, climacteric ripening of three apple cultivars, Tsugaru, Hongro and Fuji, producing different ethylene levels and ripening responses, was examined. Correspondingly in Fuji, a linear and basal ethylene level was observed over the entire period of measurements, and Tsugaru and Hongro displayed a typical climacteric rise in ethylene production. Transcript accumulation of genes involved in ethylene biosynthesis (MdACS3 and MdACO1) and MdPG1 was studied in Tsugaru, Hongro and Fuji cultivars. Expression of MdACO1 transcripts was shown in all three ripened apple fruits. However, the MdACS3 and MdPG1 were transcribed differently in these cultivars. Comparing the MdPG1 of ‘Tsugaru’, ‘Hongro’ and ‘Fuji’, structural difference was discovered by genomic Southern analysis. Overall results pointed out that MdACS3 and MdPG1 play an important role in regulation of fruit ripening in apple cultivar.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

REFERENCES

키워드

ACS3(1-aminocyclopropane-1-carboxylate synthase) gene ACO1(1-aminocyclopropane-1-carboxylate oxidase) gene PG1(Polygalacturonase) gene Ethylene pathway Apple ripening