콩 P34 단백질 결핍 유전자를 이용한 SSLP 마커 개발

• 영문명: Development of SSLP Marker Targeted to P34 Null Gene in Soybean
• 발행기관: 한국육종학회
• 저자명: 양기웅(Kiwoung Yang) 고종민(Jong-Min Ko) 이영훈(Young-Hoon Lee) 전명기(Myeong Gi Jeon) 정찬식(Chan-Sik Jung) 백인열(In-Youl Baek) 김현태(Hyun-Tae Kim) 박금룡(Keum-Yong Park)
• 간행물 정보: 『한국육종학회지』Vol.42 No.5, 502~506쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2010.10.30

영문 초록

Soybean seed possesses about 15 allergenic proteins recognized by IgEs from soy-sensitive human. The allergenic impact of soybean proteins limit its extensive usage in a broad range of processed foods. Soybean protein P34 or Gly m Bd 30k of the cysteine protease family is one of the major allergen of the soybean seed. P34-null soybean, PI567476, was identified among soybean (Glycine max & Glycine soja Sieb. and Zucc) of approximately 16,226 accessions from USDA soybean germplasm screened. Also, for P34 gene (Williams 82; whole genome sequence cultivar) and P34 null gene (PI567476) comparative analysis of sequences listed in the NCBI database showed the presence of a SSLP (Simple Sequence Length Polymorphism) of 4 base pair. So, a SSLP marker was designed to reveal the polymorphism of the locus. In this study, a population of 339 F 2 recombinant inbred lines generated by cross between Taekwang (Glycine max) and PI567476 was used to select F 2:3 plant of a P34 null gene. The result separation rate Taekwang type, heterozygous type and PI567476 type were shown in 85: 187: 67 since single gene is concerned in as the separation rate of 1:2:1 in X2 0 05 =5.99, df=2. In future, selected plant will identify protein level, whether P34 null protein is equal to P34 null gene.

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P34 null gene Soybean allergy SSLP marker MAS(Marker Assisted Selection)