Microplate법에 의한 수박 라이코핀 고함유 유전자원 선발

• 영문명: Screening of Lycopene-rich Germplasms using Microplate Method in Screening of Lycopene-rich Germplasms using Microplate Method in
• 발행기관: 한국육종학회
• 저자명: 이우문(Woo-Moon Lee) 권민정(Min Jung Kwon) 송이슬(Lee-Seul Song) 김수(Su Kim) 이희주(Hee Ju Lee) 양은영(Eun-Young Yang) 최학순(Hak-Soon Choi) 허윤찬(Yun-Chan Huh) 박동금(Dong Kum Park) 윤무경(Moo kyoung Yoon)
• 간행물 정보: 『한국육종학회지』Vol.46 No.1, 37~43쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2014.03.30

영문 초록

Recent researches focused on breeding lycopene–rich watermelon varieties are active since lycopene content in watermelon is 60% higher than in tomato. We developed a new microplate method using a UV spectrophotometer to select genetic resources of watermelon containing high lycopene content. The microplate method showed more accurate analysis (about 97% positive correlation) than HPTLC, suggesting the microplate method is a reliable analytical technique for the rapid and simple selection of watermelon resources. Lycopene contents of 96 watermelon accessions using the new microplate method was a wide variation of 3.2-98.3 ㎍.g-1FW to assess, allowing selection of 15 high-lycopene watermelon accessions. Lycopene contents of the 15 watermelon accessions was 4.4∼9.6 fold higher than lycopene content of a commercial cultivar ‘Speedplus’. These results suggest that the watermelon accessions selected using a novel microplate method can be used for breeding of lycopene-rich cultivars.

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서 언

재료 및 방법

결과 및 고찰

References

키워드

Microplate method Breeding UV spectrophotometer Watermelon Lycopene