국내 밀 품종의 2차원 전기영동 분석을 통한 고분자 글루테닌 분석

• 영문명: Two-dimensional Electrophoresis of High Molecular Weight Glutenin Subunits in Korean Wheat Cultivars
• 발행기관: 한국육종학회
• 저자명: 이종열(Jong-Yeol Lee) 박철수(Chul-Soo Park) 김효정(Hyo-Jung Kim) 김주형(Joo-Hyung Kim) 김민숙(Min-Suk Kim) 김영태(Yeong-Tae Kim) 강천식(Chon-Sik Kang) 임선형(Sun-Hyung Lim) 하선화(Sun-Hwa Ha) 안상낙(Sang-Nag Ahn) 김영미(Young-Mi Kim)
• 간행물 정보: 『한국육종학회지』Vol.45 No.3, 240~252쪽, 전체 13쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2013.09.30

영문 초록

To evaluate qualitative and quantitative expression level of HMW-GS protein, we separated glutenin fractions and conducted two-dimensional electrophoresis (2DE) in 32 Korean wheat cultivars. The average spot numbers of HMW-GS in Korean wheat cultivars were 11.78 which included 1.31, 5.53 and 4.94 spots at Glu-A1, Glu-B1 and Glu-D1 loci, respectively. Cultivars harboring 1, 2* subunits had more spots than ones with null allele in Glu-A1 locus because Glu-B1 and Glu-D1 loci did not show difference in spot number. A large variation in the total spot number of HMW-GS was observed ranging from 7 (Dahong) to 18 (Jokyung). Relative expression level of Korean wheat cultivars when compared with the check accession Chinese Spring ranged from 24% (Eunpa) to 111% (Gobun) with an average of 44%. Korean wheat accessions could be classified into six groups based on the number of HMW-GS spots and quantification value of each spot.

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키워드

HMW-GS Two-dimensional electrophoresis Relative expression level Dendrogram