미성숙 화기를 이용한 ‘우람’ 억새 식물체 재분화

• 영문명: Plant Regeneration of M. sacchariflorus cv. Wooram Using Immature Inflorescence
• 발행기관: 한국육종학회
• 저자명: 유경단(Gyeong-Dan Yu) 장윤희(Yun-Hui Jang) 김정일(Jeong-Il Kim) 이지은(Ji-Eun Lee) 안기홍(Gi Hong An) 문윤호(Youn-Ho Moon) 차영록(Young-Lok Cha) 최인후(In-Hu Choi) 안종웅(Jong Woong Ahn) 구본철(Bon-Cheol Koo) 이경보(Kyung-Bo Lee)
• 간행물 정보: 『한국육종학회지』Vol.47 No.4, 376~383쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.12.30

영문 초록

This study was carried out to establish the optimal conditions for callus induction and plant regeneration using immature inflorescence of M. sacchariflorus cv. ‘Wooram’, a bioenergy crop selected in Korea. Callus induction rate was the highest (93.3%) in MS medium containing 3 mg L-1 2,4-D, and 86.7% in MS medium containing 3 mg L-1 2,4-D combined with 0.1 mg L-1 BA. Plant regeneration rate was high when the calli derived from the medium containing BA was used, as compared with those derived from the BA-minus medium. The results showed that the medium conditions containing 5 mg L-1 BA combined with 0.1 mg L-1 NAA was the most effective in plant regeneration of which the rate reached 86.7%. The regenerated shoots were separated from the calli and roots over 3 cm were developed from the shoots after 4 week culture on basal MS medium without supplementation. The plantlets were then transferred to soil and cultured in greenhouse. After 5 weeks, the plants with the height of at least 20 cm were successfully acclimatized.

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키워드

Miscanthus Immature inflorescence Callus Regeneration