SNP 분자표지를 이용한 8배체 딸기(Fragaria × ananassa Duch.) 유전자지도 작성

• 영문명: A genetic linkage map of allo-octoploid strawberry (Fragaria × ananassa Duch.) using SNP markers
• 발행기관: 한국육종학회
• 저자명: 이예린(Ye Rin Lee) 이준대(Jundae Lee)
• 간행물 정보: 『한국육종학회지』Vol.49 No.3, 119~128쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2017.09.30

영문 초록

An allo-octoploid strawberry (Fragaria × ananassa Duch.) is one of the most important vegetable crops in Korea. However, there were few genomic researches of strawberry due to polyploidy and complexity of its genome. In this study, we aimed to construct a genetic linkage map of strawberry using single nucleotide polymorphism (SNP) markers that were developed through a next-generation sequencing (NGS) analysis. Two strawberry varieties, ‘Sulhyang’ and ‘Senga-sengana’, were used as a maternal and a paternal parent, respectively, and their F1 generation consisting of 94 individuals was used for construction of a genetic linkage map. A total of 19.0 Gbp (‘Sulhyang’) and 21.8 Gbp (‘Senga-sengana’) of genomic sequences were obtained through NGS analysis. Subsequently, approximately 87,000 SNPs were identified and 1,154 primer sets for high-resolution melting (HRM) analysis were designed through bioinformatic analysis. In result, a total of 224 polymorphic HRM markers were developed and 205 markers were mapped on the genetic linkage map of strawberry, which total length was 800.8 cM and the number of linkage groups were 30. This SNP-based genetic linkage map and the 224 SNP markers will be very helpful for the genomic and genetic researches of allo-octoploid strawberry.

목차

1. 서언

2. 재료 및 방법

3. 결과 및 고찰

4. 적요

5. 사사

키워드

Fragaria × ananassa HRM genetic map NGS SNP