Putative Secondary Structure of GroE mRNA

• 발행기관: 호서대학교 중앙도서관
• 저자명: Sung Jo Kim Han Bok Kim
• 간행물 정보: 『호서대학교 논문집』제3권, 75~80쪽, 전체 6쪽
• 주제분류: 사회과학 > 무역학
• 파일형태: PDF
• 발행일자: 1995.12.30

영문 초록

Increase of GroE protein synthesis at high temperature in Escherichia coli is known to be due to high-level transcription. Recently, regulation at translation level was proved experimentally in groE gene of Streptomyces albas (Servant et al., 1994). Whether or not secondary structures of groE mRNA of E. coli at 37 and 42°C can be distinguished was tested by using computer program, RNAdraw. When groE mRNA containing 130 nucleotides from transcription start site to +130 was analyzed, some broad base pairings from the start codon to +125 were found in the structural gene at 37°C. Its absence at 42°C might contribute to different level of GroE production. Theoretical computer analysis of mRNA secondary structures is useful in predicting and explaining different expression levels at the two temperatures.

목차

Abstract

Introduction

Materials and Methods

Results and Discussion

References