冬柏을 위시한 차나무科 植物 種實에 含有된 抗酸化劑에 관한 硏究

• 영문명: Studies on the Antioxidative Substances in the Seeds of Some Theaceae Family
• 발행기관: 한국응용과학기술학회 (구.한국유화학회)
• 저자명: 김성진(Kim Seong Jin)(金成眞) 최은진(Choi Eun Jin)(崔銀眞) 임희영(Lim Hee Ryeong)(林希玲) 김태숙(Kim Tae Sook)(金泰淑) 조용계(Joh Yong Goe)(趙鏞桂)
• 간행물 정보: 『한국응용과학기술학회지』한국유화학회지 제8권 제1호, 35~43쪽, 전체 9쪽
• 주제분류: 공학 > 화학공학
• 파일형태: PDF
• 발행일자: 1991.05.30

영문 초록

Dried seeds of Camellia japonica and Thea sinensis were investigated to determine the nature of their antioxidative activity. Activity was measured by the induction period in the coupled oxidation of a substrate lard and extracts or isolates to be tested. 70% methanol and dichloromethane extracts were found to be antioxidative abilities. Their unsaponifiables revealed weak antioxidative activity, although hexane extracts did not show antioxidative effect on lard. Column chromatography for dlchloromethane extracts gave 4 fractions(only 2 fractions were potent). HPLC was used in isolating potent antioxidative components from the column fractions and the precolumn-passed methanol extracts. They were separated into 7 and 8 components, respectively. The column fractions obtained from both seeds comprised trans-p-coumaric acid. trans-p-ferulic acid and an unknown component with minor components such as chlorogenic acid and catechin. On the other hand, the most prominent components in the methanol extracts were an unidentified component. trans-pcoumaric acid, trans-p-ferulic acid, catechin and chlorogenic acid. The unknown compound isolated from the column fractions and methanol extracts was identified as epicatechin by ¹H-and ¹³C-NMR. The antioxidative activities of these components were epicatechin > catechin > chlorogenic acid > trans-p-ferulic acid > trans-p-coumaric acid.

목차

ABSTRACT

I. 序論

II. 材料 및 實驗方法

III. 結果 및 考察

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