세포막 물질수송계의 조절에 의한 유기수은의 세포독성 변화에 대한 연구

• 영문명: Changes on the Methylmercury-induced Cytotoxicity by Control of Cell Membrane Transport System
• 발행기관: 한국환경보건학회
• 저자명: 염정호 고대하 김준연 김남송
• 간행물 정보: 『1. 한국환경보건학회지』제26권 제4호, 88~96쪽, 전체 9쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2000.12.30

영문 초록

The aim of the current study was to evaluate the transport system in EMT-6 cell for the uptake of the methylmercury(MeHg). Several inhibitors ere used to test used to test which potential transport system might be involoved in MeHg uptake. Probenecid was used to test the organic transport system, valinomycin for testing the effect of the membrane potential, cytochalasin B for testing the facilitated diffusive D-glucose transport system and colchicine for testing the microtubule system. Ouabain for evaluating active transport system, 4 ,4-diisothiocyano-2 ,2-stilbenedisulfonic acid(DIDS) the Cl- ion transport system and verapamil for the Ca2+ transprot system. Significantly, MeHg decreased the synthesis of nitric oxcide(NO) and intracellular ATP in ENT-6 cells. In the condition of ouabain containing with MeHg decreased the production of NO and intracelluar ATP. In the treatment of inhibitors, ouabain showed protective effect against cytotoxicity of MeHg but ather inhibitors not showed protective effects. The protective effects of ouabain against the cytotoxicity of MeHg deoended on the concentration of added ouabain to the culture medium for MET-6 cells. These result showed that the uptake of MeHg might be involved in the active transport system. Active transports system seems to share similarities with the transport systems for the uptake of MeHg when using MeHg and MeHg-glutathione complex.x.

키워드

MeHg Ouabain Active transport