구강 편평세포암종에서 p53 단백과 증식성 세포핵항원 발현에 대한 면역조직화학적 연구

• 영문명: An IMMUNOHISTOCHEMICAL STUDY OF p53 PROTEIN AND PROLIFERATING CELL NUCLEAR ANTIGEN EXPRESSION IN ORAL SQUAMOUS CELL CARCINOMA
• 발행기관: 대한구강악안면외과학회
• 저자명: 이진환(Jin Hwan Lee) 유선열(Sun Youl Ryu)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제24권 제4호, 411~420쪽, 전체 10쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 1998.10.30

영문 초록

The present study was aimed at investigating the usefulness of clinical stage, degree of histopatphological malignancy, p53 protein and PCNA labeled indices in deciding the prognosis, proliferation and infiltration of oral squamous cell carcinoma (SCC). Twenty-seven cases of SCC (seven were recurrent cases) were analyzed and 10 cases of non-specific inflammatory lesion served as control. Malignant scores were obtained with the Anneroth s classification. Proliferative activity of SCC in oral cavity was examined by means of proliferaling cell nuclear antigen (PCNA) and p53 protein immunostaining using DO7, PC10 (Novostra Co., U.K.) as primary antibody and biotinylated linked antibody (LSAB kit, Dako Co., U.S.A.) as secondary antibody. The labeled indices of PCNA and p53 were calculated by computer image analysis system in 400 multiplication microscopic fields. Results obtained were as follows; 1. The mean age of SCC group was 61.2 years. Seven of 20 cases had recurrence in 4 months after the operation, in which no sigificant correlations were noted between pre- and postoperative clinical stage, malignant scores, p53 protein and PCNA indices. 2. Positive immunostaining was noted in 65% (13 of 20 cases) on p53 protein and in 80% (16 of 20 cases) on PCNA. 3. Mean p53 protein and PCNA labeled indices were 24.71±27.86% and 41.49±2.51% in SCC group. They were high in SCC group than those in control group, although there was no signficant correlation between the two indices. 4. Mean malignant score was 15.22 0.64 in SCC group. There was no correlation between malignant score and clinical stage, and between malignant score and p53 protein or PCNA indices. 5. There was no signficant correlation between SCC and recurrent SCC groups in malignant scores, p53 protein or PCNA indices. These results suggest that p53 and PCNA labeled indices have only limited usefulness in deciding the prognosis, proliferation and infiltration of oral squamous cell carcinoma

목차

Abstract

I. 서론

II. 연구재료 및 방법

III. 연구결과

IV. 고찰

V. 결론

참고문헌

키워드

squamous cell carcinoma immunohistochemical study