구강 편평세포암종에서 Differential Polymerase Chain Reaction에 의한 Cyclin D1 유전자의 증폭에 대한 연구

• 영문명: CYCLIN D1 GENE AMPLIFICATION IN ORAL SQUAMOUS CELL CARCINOMA USING DIFFERENTIAL POLYMERASE CHAIN REACTION
• 발행기관: 대한구강악안면외과학회
• 저자명: 김기순(Kee Soon Kim) 김경욱(Kyung Wook Kim) 이재훈(Jae Hoon Lee) 김창진(Chang Jin Kim)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제26권 제4호, 355~362쪽, 전체 8쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2000.08.30

영문 초록

Neoplastic growth is characterized by alterations of oncogenes and antioncogenes. The interaction between activated oncogenes and functional deletion of antioncogene appears to be the driving force directing normal cells to uncontrolled growth resulting in tumor. In addition to those genes mentioned, other genes controlling the entry of cells into the cell cycle have recently been implicated in cancer development. The overexpression of the cyclin D1 gene, which has been mapped to 11q13, either by gene rearrangement or amplification has been noted in various malignant tumors. The product of the cyclin D1 gene forms a complex with cyclin-dependent protein kinases(CDK4) that governs a key transition in the cell cycle. The relationships between the overexpression of cyclin D1 assessed by immunihistochemistry and the amplification of the cyclin D1 gene by differential polymerase chain reaction(DPCR) using primers for dopamin D2 receptor gene in 13 cases of squamous cell carcinomas of the oral cavity have been studied. The semiquantitative assay of cyclin D1 amplification has been made by cyclin D1/dopamin D2 receptor(CD/DR) ratio. The results were as follows; 1. In the normal tissue and the tumor, the CD/DR ratios were 0.82 and 1.36 respectively. This implicates 1.65-fold amplification of cyclin D1 gene in tumor compared to that in normal tissue. 2. The tumor tissue which showed overexpression of cyclin D1 by immunohistochemistry revealed 2-fold amplification of cyclin D1 compared to the normal tissue. 3. The tumor tissue which showed mild expression of cyclin D1 by immunihistochemistry revealed 1.7-fold amplification of cyclin D compared to the normal tissue. 4. The cyclin D1 was overexpressed in the tumor tissue at the rate of 38%. Above results suggest that cyclin D1 has close correlation with the development of carcinoma in the oral cavity. But further studies were needed to elucidate the carcinogeneic mechanisms by comparative studies among cyclin D1, pRb and p53.

목차

Abstract

Ⅰ. 서론

Ⅱ. 연구재료 및 방법

Ⅲ. 연구결과

Ⅳ. 총괄 및 고찰

Ⅴ. 결론

참고문헌