세균 독소를 작용시킨 섬유아 세포에서 Transforming Growth Factor-β₁의 생성

• 영문명: PRODUCTION OF TRANSFORMING GROWTH FACTOR-β₁ IN HUMAN FIBROBLASTS INDUCED WITH BACTERIAL TOXINS
• 발행기관: 대한구강악안면외과학회
• 저자명: 이성근(Seong Geun Lee) 김광혁(Kwang Hyuk Kim)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제26권 제4호, 345~354쪽, 전체 10쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2000.08.30

영문 초록

TGF-β₁ is a potent chemotactic factor for inflammatory cells and fibroblasts. It also stimulates the celluar source and components of extracellular matrix and the production of proteinase inhibitors. Collectively, these biologic activities lead to the accumulation and stabilization of the nascent matrix, which is vital to wound healing. The objective of this study is to investigate production of TGF-β₁ in vitro fibroblast culture in the presence of Staphylococcus enterotoxin B(SEB) and/or lipopolysaccharide(LPS) and to elucidate the role of TGF-β₁ which may be responsible for wound healing The fibroblasts were originated from facial dermis and hypertrophic scar in 26 year-old male patient. In the presence of LPS(0.0l㎍, 0.1㎍, 1.0㎍), SEB(0.0l㎍, 0.1㎍, 1.0㎍) respectively, cells(5×103ml) were cultivated in vitro. At 1, 3, and 5 days after incubation, cells were counted. Also, cells(2.5×105ml) were cultivated in EMEM with LPS(0.01, 0.1 and 1.0㎍), SEB(0.01, 0.1 and 1.0㎍) respectively and LPS(0.1㎍) and SEB(0.1㎍) in combination for 24, 48, and 72 hours respectively. Culture supernatants were harvested at 1, 2, and 3 days after incubation period and triplicate culture supernatants were pooled and TGF-β₁ was assayed in duplicate. The results were as follows. 1. In facial dermal fibroblast induced with SEB and LPS respectively or in combination, the suppression of cell proliferation occurred very significantly at 1 day after incubation, compared with the control. In SEB exposure, the production of TGF-β₁ was decreased very significantly at 1 day after incubation, compared with the control. However, in LPS, SEB and LPS exposure, the production of TGF-β₁ was increased very significantly at 1 day after incubation, compared with the control. 2. In hypertrophic scar fibroblast induced with SEB and LPS respectively or in combination, the suppression of cell proliferation did not occur at 1 day after incubation, compared with the control. In SEB and LPS exposure in combination, the production of TGF-β₁ was increased very significantly at 1 day after incubation, compared with the control. However, the production of TGF-β₁ did not occur in SEB and LPS exposure respectively. In conclusion, the concentration of bacterial toxins and the incubation period correlated with cell proliferation and production of TGF-β₁ very significantly and both fibroblasts have different phenotype each other in this regard. This data suggest that the significant production of TGF-β₁ may develope abnormal wound healing associated with tissue fibroproliferative disorder, such as hypertrophic scar and keloid formation.

목차

Abstract

Ⅰ. 서론

Ⅱ. 연구재료 및 방법

Ⅲ. 연구결과

Ⅳ. 총괄 및 고찰

Ⅴ. 결론

참고문헌

키워드

Wound healing Transforming growth factor-β1 Lipopolysaccharide