구강종양에서 p53 종양억제 유전자의 돌연변이에 관한 연구

• 영문명: A STUDY ON MUTATIONS OF P53 TUMOR SUPPRESSOR GENE IN ORAL TUMORS
• 발행기관: 대한구강악안면외과학회
• 저자명: 주성채(Seong chai Joo) 표성운(Sung woon Pyo)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제26권 제1호, 45~52쪽, 전체 8쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2000.02.28

영문 초록

Nowadays, there are a lot of evidence that mutation of the p53 tumor suppressor gene is one of the most common genetic abnormalities in neoplastic progression. In this study, we analyzed 20 specimens of oral tumors(squamous cell carcinoma 14 cases, ameloblastoma 3 cases, adenoid cystic carcinoma 2 cases, malignant schwannoma 1 case)using polymerase chain reaction and direct sequencing which used an automated DNA sequencer and software for detection of mutations. Polymerase chain reactions were performed with 4 sets of primers encompassing exon 5, 6, 7, 8, and direct sequencing method was employed. The results were as followings. 1. We detected 10 piont mutations out of 20 specimens (50%). 2. The genetic alterations included 7 mis-sense mutations resulting in single amino acid subtitutions, 2 silent mutations, 1 non-sense mutations encoding a stop codon. 3. Mutations were mostly in exon 7(7 out of 10 mutations, 70%) and involved codons 225, 234, 235, 236, 238, 247. 4. Therse were 4 cases of T→A transversion, 2 cases of C→A transversion, A→G transition, 1 case of C→G, T→G transversion respectively. 5. We could find out point mutations more conveniently using PCR-Automated Direct Sequencing method.

목차

Abstract

Ⅰ. 서론

Ⅱ. 연구재료 및 방법

Ⅲ. 연구결과

Ⅳ. 총괄 및 고찰

Ⅴ. 결론

참고문헌

키워드

Oral tumor p53 gene Point mutation Polymerase chain reaction Automatic direct sequencing method