ELISA-LC/MS/MS 병행에 의한 식품 중 aflatoxins 분석

• 영문명: Determination of Total Aflatoxins in Foods by Parallelism of ELISA and LC/MS/MS
• 발행기관: 한국환경보건학회
• 저자명: 김경열(Kyeongyeol Kim) 남민지(Minji Nam) 남보람(Bo-Ram Nam) 류희정(Hee-Jung Ryu) 송정언(Jeong-Eon Song) 심원보(Won-Bo Shim) 이수형(Soo-Hyung Lee) 정덕화(Duck-Hwa Chung)
• 간행물 정보: 『1. 한국환경보건학회지』제36권 제1호, 52~60쪽, 전체 9쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2010.02.28

영문 초록

High performance liquid chromatography (HPLC) and liquid chromatography mass spectrometry (LC/MS) have been widely used to quantify aflatoxins in food, but these methods are expensive, time-consuming, unsuitable for analysis of the routine screening of large sample numbers and require derivatization and high level techniques to perform. The objective of this study is to detect aflatoxins in a large number of foods by a high efficient analytical system of combined enzyme linked immunosorbent assay (ELISA) for screening and LC/MS/MS for confirmation. The samples spiked individually with aflatoxin B1 (0.5 and 1.0 ng/g) and total aflatoxins (10 ng/g) were analyzed by ELISA and LC/MS/MS, and the recoveries for ELISA and LC/MS/MS were 71.8~119.2% and 70.8~135.3%, respectively. A total of 378 samples (grains, nuts, soybean and fermented soybean foods, pepper and fermented pepper foods) were purchased from the six major cities in Korea and analyzed by ELISA-LC/MS/MS system. Twenty two (5.8%; peanut: 11, pistachio: 2, walnut: 6, almond: 1, pepper powder: 1, pepper paste: 1) out of 378 samples were screened as aflatoxin B1 positive by ELISA, but, 4 (1.1%; peanut: 2, pistachio:1, pepper powder: 1) out of the 22 samples screened were confirmed as aflatoxins positive at levels of 1.02~52.79 ng/g by LC/MS/MS. ELISA-LC/MS/MS system provides a more rapid, accurate and cost-effective method for the detection of aflatoxins in large number of samples.

목차

I. 서 론

II. 연구방법

III. 결과 및 고찰

IV. 결 론

참고문헌

키워드

simultaneous aflatoxin ELISA LC/MS/MS monitoring