LC/MS/MS를 이용한 산화성 스트레스 지표로써 소변 중 8-iso-PGF2α 분석

• 영문명: Determination of 8-iso-PGF2α as Oxidative Stress Marker in Human Urine by High Performance Liquid Chromatography with Tandem Mass Spectrometry
• 발행기관: 한국환경보건학회
• 저자명: 고영림(Young Lim Kho) 이은희(Eun Hee Lee) 채홍재(Hong Jae Chae) 최경호(Kyungho Choi) 백도명(Domyung Paik)
• 간행물 정보: 『1. 한국환경보건학회지』제36권 제1호, 44~51쪽, 전체 8쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2010.02.28

영문 초록

This study aimed to develop analytical method for 8-isoprostanes as biomarkers for oxidative stress with LC/MS/MS technique and to apply the method for human urine samples. Analyzed compounds for urinary oxidative stress markers were 7 stereo-isomers of prostaglandins and the internal standard (iso-PGF2α-d4) was used to adjust the recovery rate. The method for determining urinary iso-PGF2α consisted of solid phase extraction and LC/MS/MS detection. Separation of isomers of prostaglandins completed by porous graphitic carbon column and buffer solution. Detection limits for urinary markers of oxidative stress, iso-PGF2α with LC/MS/MS were 0.01 ng/ml by S/N ratio 3 and 0.028 ng/ml by calculated as to FDA method. The recovery (92.8~101.9%) and precision (8.8~20.7%) of analysis were feasible for detecting iso-PGF2α in real human urine samples. We detected 4 isomers of prostaglandins in human urine samples. Mean (standard deviation) of urinary iso-PGF2α concentration were 0.231 (0.117) ng/mg creatinine for smoking group and 0.154 (0.082) ng/mg creatinine for non-smoking group.

목차

I. 서 론

II. 연구방법

III. 결과 및 고찰

IV. 결 론

키워드

oxidative stress 8-iso-PGF2α prostaglandin LC/MS/MS