파이로시퀀싱을 이용한 한강상수원보호구역 수계 중의 세균 다양성

• 영문명: Analysis of Bacterial Diversity in Water from the Han River Water Source Protection Area via a Pyrosequencing Assay
• 발행기관: 한국환경보건학회
• 저자명: 김희정(Heejung Kim) 권덕인(Dugin Kaown) 김창수(Changsoo Kim) 이시원(Siwon Lee)
• 간행물 정보: 『1. 한국환경보건학회지』제42권 제4호, 274~279쪽, 전체 6쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2016.08.30

영문 초록

Objectives: We investigated bacterial diversity in the Han River water resource protection area in order to provide basic microbiological information on the drinking water safety of the Seoul metropolitan region. Methods: Samples were collected in the spring and winter, but not during the rainy season. Pyrosequencing, gene amplification, and extraction of nucleic acids were employed in this study. Results: In total, 57 and 48 operational taxonomic units were respectively analyzed in samples collected during spring and winter. Proteobacteria were predominant in all samples. The samples contained phylogenetically diverse bacterial communities, with eleven major phyla and 36 genera. Cyanobacteria were predominant in the spring samples, but not in the winter samples. The predominant species in the samples collected during both seasons belonged to the genus Aquamicrobium and Bradyrhizobium. Moreover, no pathogenic bacteria were detected in the samples. Conclusion: Proteobacteria were predominant in the samples from the Han River water source protection area. Cyanobacteria were more predominant in the spring samples than in the winter samples, but Aquamicrobium and Bradyrhizobium were predominant in both sampling seasons.

목차

I. 서 론

II. 재료 및 방법

III. 결 과

V. 고 찰

V. 결 론

키워드

Drinking water safety Han River Pyrosequencing water resource protection area