자몽종자추출물, 구연산, 사과산 그리고 염화벤잘코늄을 주성분으로 하는 스프레이형 소독제의 조류인플루엔자바이러스에 대한 살바이러스 효과

• 영문명: Virucidal Efficacy against Avian Influenza Virus of a Disinfectant Spray Containing Grapefruit Seed Extracts, Citric Acid, Malic Acid and Benzalkonium Chloride
• 발행기관: 한국환경보건학회
• 저자명: 차춘남(Chun-Nam Cha) 박은기(Eun-Kee Park) 정지윤(Ji-Youn Jung) 유창열(Chang-Yeol Yoo) 김석(Suk Kim) 이후장(Hu-Jang Lee)
• 간행물 정보: 『1. 한국환경보건학회지』제42권 제4호, 266~273쪽, 전체 8쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2016.08.30

영문 초록

Objectives: This study evaluated the virucidal efficacy against avian influenza virus (AIV) of a disinfectant spray containing 0.25% grapefruit seed extract, 0.2% citric acid, 0.0625% malic acid and 0.0125% benzalkonium chloride. Methods: The disinfectant spray was diluted several times with hard water (HW) and organic matter (OM). Two point five mL of each diluent was added into each test tube, and 2.5 mL of AIV suspension was inserted into each test tube. After 30 minutes of virus-disinfectant contact reaction at 4 o C, 2.5 mL of 10% inactivated fetal bovine serum was added into each test tube to neutralize the sanitizer efficacy. The neutralized solutions were serial 10-fold dilutions with phosphate buffer solution, and 0.2 mL of the diluents was injected into the allantoic cavity of five ten-day-old-chickens per dilution time. After incubation of the embryos for five days, the viability of the AIV was examined by hemagglutination titer. The valid dilution of the disinfectant spray was estimated according to the dilution time that the virus titer was inactivated more than 10 4 50% egg-infective dose (EID50)/ mL compared with pathogen control. Results: In HW and OM conditions, the valid dilutions of the disinfectant spray against AIV were seven- and three-fold dilutions, respectively. The AIV titer of the pathogen control was more than 6.1 log10EID50/mL, and there was no embryonic toxicity. Conclusion: The present study showed that this disinfectant spray has effective virucidal activity against AIV.

목차

I. 서 론

II. 재료 및 방법

III. 결과 및 고찰

IV. 결 론

키워드

Avian influenza virus benzalkonium chloride citric acid disinfectant spray grapefruit seed extracts