구강 편평세포암에서 E-cadherin 유전자의 과메틸화

• 영문명: THE HYPERMETHYLATION OF E-CADHERIN GENE IN ORAL SQUAMOUS CELL CARCINOMA
• 발행기관: 대한구강악안면외과학회
• 저자명: 표성운(Sung-Woon Pyo) 김영실(Young-Sill Kim) 박지영(Ji-Young Park) 김창현(Chang-Hyen Kim) 이원(Won Lee) 박민규(Min-Kyu Park)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면회과학회지 제34권 제2호, 135~140쪽, 전체 6쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2009.04.30

영문 초록

Loss of E-cadherin (E-cad) expression has been found in multiple cancers and is postulated to facilitate tumor cell dissociation and metastais. Promotor methylation may provides an alternative pathway for loss of gene function. This study evaluated the role of hypermethylation in the downregulation of E-cad in oral squamous cell carcinoma (OSCC). We examined the E-cad expression by immunohistochemical staining and detected methylation status by methylation-specific polymerase chain reaction (MSP) in 20 OSCC tissues. Overally, 12 (60%) cases of hypermethylation of Ecad were detected and we found there were no correlation between methylation and age, histologic grade, lympn node metastasis, tumor size and clinical stage. However, Eleven (73.3%) of 15 samples which was negative for E-cad staining showed hypermethylation of E-cad promotor region. On the other hand, only one (20%) of 5 E-cad positive sample was observed with methylated status. The underexpression of E-cad was found to be related to promotor hypermethylation (p=0.035). In conclusion, we suggest that hypermethylation play a role in inactivation of E-cad gene and may be a appreciable biomarker for diagnosis and treatment of OSCC.

키워드

Oral squamous cell carcinoma E-cadherin Hypermethylation Methylation-specific PCR