Alkaline Inducing Agent 및 Alkaline Proteolytic Enzyme 혼용처리에 의한 Shaving Scraps 가수분해 단백질의 제조 및 특성

• 영문명: Preparation and Characterization of the Hydrolyzed Protein from Shaving Scraps of Leather Waste Containing Chromium by the Combination Treatment with Alkaline Inducing Agent and Alkaline Proteolytic Enzyme
• 발행기관: 유기성자원학회
• 저자명: 김원주(Won-Ju Kim) 조주식(Ju-Sik Cho) 이홍재(Hong-Jae Lee) 허종수(Jong-Soo Heo)
• 간행물 정보: 『유기물자원화』제6권 제1호, 1~12쪽, 전체 12쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 1998.06.30

국문 초록

피혁제조시 발생되는 크롬을 함유한 피헥 고형폐기물인 shaving scrap의 단백질 자원화 가능성을 검토하기 위하여 MgO를 기본으로 하여 alkaline inducing agents 벚 alkaline proteolytic enzymes을 흔용처리하여 shaving scrap으로 부터 회수한 가수분해 단백질의 용해도，무기성분 함량，분자량분포 등을 비교 검토함으로서 최적 가수분해 조건 벚 액체비료의 원료로 활용하기 위한 저분자 단백질의 회수방안을 조사한 결과는 다음과 같다. Alkaline inducing agents의 혼용처리에 의한 shaving scrap의 가수분해 실험결과 7% MgO를 기본으후 하여 alkaline inducing agents 종류에 따라 65-85% 범위로 용해도 차이가 뚜렸하였으며，가수분해되는 정도는 NaOH>Ca(OH)2> KOH순으로 나타났으며， 획득된 hydrolyzed protein의 평균분자량은 NaOH처리시 약 10 KD, Ca(OHh 처리시 약 40KD, KOH처리시 약 80KD 이었으며，크롬함유량은 약 15ppm 이었다. Alkaline proteolytic enzymes의 혼용처리에 의한 shaving scrap의 가수분해 실험결과 alkaline proteolytic enzymes 종류에 따라 Alcalase> Esperase > Savinase순으로 용해도 차이를 보였으며, O. 5% Alcalase의 처리에 의해 용해도 85%수준，평균분자량 1KD 미만，크롬 함유량 10ppm 이하인저분자 형태의 hydrolyzed protein을 획득할 수 있었다.

영문 초록

To examine the possibility of protein recyding of shaving scraps containing chromium generated from manufacturing process of leather, the optimum hydrolysis conditions and the withdrawal methods of low molecular weight protein for using the liquid fertilizer sources by investigation of solubilities of hydrolyzed protein, inorganic nutrients contents and molecular weight distributions of hydrolyzed protein from shaving scraps treated with mixed alkaline inducing agents and mixed alkaline proteolytic enzymes induding MgO were investigated. 1n hydrolysis of shaving scraps treated with mixed alkaline inducing agents, the solubility of shaving scraps were dearly different with 65-85% according to the sorts of the inducing agents, and the degree of hydrolysis Was high in the order of NaOH, Ca(OH)2 and KOH. The average molecular weights of withdrawal hydrolyzed protein were 10, 40 and 80 KD treated with NaOH, Ca(OHh and KOH, respectively. And the chromium contents was about 15 ppm. In hydrolysis of shaving scraps treated with mixed alkaline proteolytic enzymes, the bility of shaving scraps were high in the order of alcalase, esperase and savinase. In c of treating 0.5% alcalase, the low molecular weight of hydrolyzed protein could be withdrawn. The solubility of the hydrolyzed protein was about 85%, the average molecular weight of the protein was below 1 KD and chrome content of the protein was below 10 ppm.

목차

1. 서 론

2. 재료 및 방법

3. 결과 및 고찰

4. 결 론

키워드

shaving scrap alkaline inducing agents alkaline proteolytic enzymes 가수분해 단백질 용해도 분자량 분포 shaving scrap alkaline inducing agents alkaline proteolytic enzymes solubility hydrolyzed protein molecular weight.