후두암 세포주에서 TGF-β1에 의한 MMP2와 MMP9의 발현 양상

• 영문명: The Effect of Transforming Growth Factor-β1 on Expression of MMP 2 and MMP 9 Cell Lines
• 발행기관: 대한두경부종양학회
• 저자명: 권남영(Nam Young Kwon) 김형진(Hyung Jin Kim) 우정수(Jeong Su Woo) 권순영(Soon Young Kwon) 정광윤(Kwang Yoon Jung)
• 간행물 정보: 『대한두경부종양학회지』제18권 제2호, 135~141쪽, 전체 7쪽
• 주제분류: 의약학 > 종양학
• 파일형태: PDF
• 발행일자: 2002.11.30

영문 초록

Backgrounds and Objectives: Metastasis is a complex multistep process that requires sequential interactions between the invasive cell and the extra-cellular matrix. Transforming growth factor-β1 (TGF-β1) is a multifunctional regulator of cellular differentiation, motility and growth. Loss of sensitivity to the growth inhibitory effects by TGF-β1 plays important roles in neoplastic progression. The aim of this study was to investigate the role of TGF-β1 in the neoplastic invasion and metastasis through matrix metalloproteinase (MMP) of laryngeal cancer cell lines. Material and Methods: Two laryngeal cancer cell lines, SNU-899 and SNU-1076 were treated with recombinant TGF-β1 , and the expression of MMP-2 and MMP-9 was immunohistochemically evaluated and gelatinase activity was studied by gelatin zymogram. Results: The cell growth inhibition was evident on 4th days after 1ng/ml and 10ng/ml TGF-β1 treatment. The expressions of MMP-2 and MMP-9, and their gelatinase activities were increased in dose-dependent manner. Conclusion: TGF-β1 treatment in laryngeal cancer cell lines induces the expression of MMP-2 and MMP-9, thus playing a role in the digestion of extracellular matrix gelatin.

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키워드

Laryngeal neoplasms Metastasis TGF-β1 MMP2 MMP9