정제봉독의 멜라닌 생성 억제 효과

• 영문명: Inhibitory Effects of Purified Bee Venom on Melanin Synthesis
• 발행기관: 대한약학회
• 저자명: 한상미(SangMi Han) 김정민(JungMin Kim) 이광길(KyungGill Lee) 박관규(KwanKyu Park) 장영채(YoungChae Chang)
• 간행물 정보: 『약학회지』제56권 제4호 (2012년), 254~259쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2012.08.31

영문 초록

To further access honeybee (Apis mellifera L.) venom (BV) as a cosmetic ingredient and potential external treatment for topical use, we investigated its ability to inhibit tyrosinase activity and melanin biosynthesis on melanogenesis in B16F1 melanoma cells. We found that BV increased the cell viability in B16F1 melanoma cell and BV (0.01~1 μg/ml) inhibited melanin synthesis in with 10 nM α-melanocyte-stimulating hormone (α-MSH) for 48 h. In addition, we used reverse transcription-polymerase chain reaction and western blotting for me melanogenesis-related genes such as tyrosinase to examine the mechanisms underlying the inhibitory effects of BV on melanogensis. BV inhibited direct tyrosinase activity, which decreased melanin synthesis in α-MSH stimulated B16F1 melanoma cells. Thease findings suggest that BV induces the downregulation of melanogenesis by inhibiting tyrosinase activation.

키워드

bee venom B16F1 melanoma cell melanin tyrosinase