역기하렌즈와 RGP렌즈 착용 후 가토각막에서 상피세포의 이동과 재분포에 관한 고찰

• 영문명: The Effect of RGP Lens and Reverse Geometry Lens on Redistribution of Corneal Epithelial Cell in Rabbit
• 발행기관: 대한안과학회
• 저자명: 정용진 이하범 박성표,Yong Jin Jeong, MD, Ha Bum Lee, MD, Sung Pyo Park, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume55,number1, 110~118쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2014.01.15

영문 초록

Purpose: To investigate the migration and redistribution of rabbit corneal epithelial cells when wearing reverse geometry lens (RGL) or rigid gas permeable lens (RGP). Methods: In 30 rabbits, the right eyes were fitted with either RGL or RGP and the left eyes were untreated to serve as controls. The rabbits were sacrificed at 1, 3, 7, 10 and 14 days after lens fitting. The central and peripheral corneal thicknesses were measured by microscope and the ratio of right to left corneal thickness was calculated to evaluate the characteristics of change over time. By using the molecular probe 7-nitrobenz-2-ox-1,3-diazolylphallacidin (NBD phallacidin), the samples were examined with light microscope to determine the migration and redistribution of epithelial cells in the rabbit cornea. Results: No consistent changes in the thickness of both central and peripheral corneal epithelium were found. The corneal epithelial cells of both eyes with RGL and RGP reacted positively to NBD phallacidin. The fluorescence was most increased at day 3 of sacrifice in RGL cases and at day 7 in RGP cases, and then decreased in both cases. The corneal epithelium of eyes with RGL exhibited marked increase in the intensity of fluorescence compared to the eyes with RGP. Conclusions: The corneal epithelium with RGL showed the strongest intensity of NBD phallacidin fluorescence. This result suggests that wearing RGL may induce the migration and redistribution of corneal epithelial cells. J Korean Ophthalmol Soc 2014;55(1):110-118

키워드

Corneal epithelial cell, Redistribution, Reverse geometry lens, 7-nitrobenz-2-ox-1,3-diazolylphallacidin (NBD phallacidin)