산소로 유도된 망막증 모델에서 트리암시놀론이 망막 혈관 관련 인자에 미치는 영향

• 영문명: Effect of Triamcinolone on Retinal Vessel-Related Factors in Oxygen-Induced Retinopathy Rats
• 발행기관: 대한안과학회
• 저자명: 유웅선 서성욱 박종문 유지명 정인영,Woong Sun Yoo, MD, Seong Wook Seo, MD, PhD,, Jong Moon Park, MD, PhD,,Ji Myong Yoo, MD, PhD,, In Young Chung, MD, PhD,
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume53,number12, 1864~1869쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2012.12.15

영문 초록

Purpose: The present study investigated the effects of triamcinolone acetonide (TA) on retinal vessel-related factors and retinal vascular leakage in the retina of oxygen-induced retinopathy (OIR) rats. Methods: Sprague-Dawley rats used for OIR were exposed to hyperoxia from postnatal day 2 to day 14, then returned to normoxia from day 15 to day 30 and compared with control rats. On postnatal day 16, 2 μl of TA (4 mg/kg) was injected into the vitreous of the right eye in control and OIR rats. The Evans blue method was used for evaluating vascular leakage and RT-PCR was performed for confirmation of mRNA expression. Results: In OIR rats exposed to hyperoxia, retinal vascular permeability increased when returned to normoxia. After intravitreal injection of TA, vascular permeability was decreased in OIR rats, but no changes were found in control rats. In OIR rats, mRNAs of HIF-1α, VEGF, SDF-1 and ICAM-1 were more expressed and down-regulated after intravitreal injection of TA. Occludin mRNA level was decreased in the hypoxic condition and up-regulated after TA treatment. Conclusions: The present study suggests the ability of TA to inhibit retinal vascular leakage in OIR rats and a possibility that TA controls expression of the HIF-1, VEGF, SDF-1, ICAM-1 and Occludin, which may protect retinal vascular destruction from hypoxic conditions; further studies are necessary to confirm changes in protein levels.

키워드

Hypoxia inducible factor, Oxygen-induced retinopathy (OIR), Triamcinolone acetonide, Vascular endothelial growth factor