원자현미경을 이용한 마이토마이신 노출 시간에 따른 공막 표면 콜라젠의 변화 관찰

• 영문명: Effects of Mitomycin C on Scleral Collagen Fibrils According to Atomic Force Microscopy
• 발행기관: 대한안과학회
• 저자명: 이희재 최삼진 정유진 정경복 진경현 박헌국 이승준,Hui-Jae Lee, MD, Samjin Choi, PhD, Youjin Cheong, MD, PhD Candidate, Gyeong Bok Jung, PhD, Kyung-Hyun Jin, MD, Hun-Kuk Park, MD, PhD,, Seung Jun Lee, MD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume52,number6, 671~678쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2011.06.15

영문 초록

Purpose: To investigate the effects of mitomycin C on the scleral collagen surfaces using atomic force microscopy (AFM). Methods: Two non-contact mode AFM machines were used to observe changes in the morphological characteristics of human scleral surfaces before and after one, three, and five minutes of 0.02% mitomycin C application. Based on AFM topography and deflection images of the collagen fibril, the morphological characteristics of scleral fibrils including the fibril diameter and D-period were measured using the line profile. Results: The sclera collagen fibril treated with 0.02% mitomycin C for one minute did not show any significant increases in mean fibril diameter (155.04 ± 17.46 nm) or mean D-periodicity (70.02 ± 3.33 nm), compared to those of the control group. However, the scleral collagen fibrils treated with 0.02% mitomycin C for three and five minutes showed significant increases in mean fibril diameter (182.33 ± 16.33 nm, 199.20 ± 12.40 nm, respectively) and mean D-periodicity (70.27 ± 13.66 nm, 72.75 ± 19.32 nm, respectively), compared to those of the control group. Conclusions: The present study examined the structural changes in the scleral collagen fibrils before and after mitomycin C application according to atomic force microscopy. The results indirectly suggest that three or more minutes of 0.02% mitomycin C application affects the morphology of scleral collagen. J Korean Ophthalmol Soc 2011;52(6):671-678

키워드

Atomic force microscopy, Collagen fibrils, Diameter, D-banding, Human sclera, Mitomycin C