안와섬유모세포의 지방세포 분화에 대한 혼합 갱글리오사이드의 영향

• 영문명: Effect of Gangliosides Mixture on Differentiation of Orbital Fibroblasts into Adipocytes
• 발행기관: 대한안과학회
• 저자명: 최윤희 조은형 국경훈 ,Youn-Hee Choi, MD, PhD,, Eun Hyung Cho, MD, Koung Hoon Kook, MD, PhD,
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume52,number3, 338~344쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2011.03.15

영문 초록

Purpose: To investigate the role of gangliosides in the differentiation of orbital fibroblasts into adipocytes, a component in the pathogenesis of Graves’ ophthalmopathy. Methods: Orbital tissues were obtained during orbital surgery for subjects without Graves’ ophthalmopathy or other inflammatory orbital disease, and orbital fibroblasts were primarily cultured from each obtained tissue. Morphological examination of orbital fibroblasts was performed after treatment with commercially available gangliosides mixture (Gmix) comprised of several subtypes. To determine the effect of Gmix on the differentiation of orbital fibroblasts into adipocytes and the differentiation-related genes, Oil Red-O staining and RT-PCR were performed. Results: The treatment with Gmix induced the morphological changes, which at least in part were explained with the differentiation of orbital fibroblasts into adipocytes in accordance with the increase of mRNA level of genes known to be related to adipogenesis, whereas dermal fibroblasts and preadipocytes were irresponsive to the same treatment. Conclusions: The results from the present study suggest gangliosides may have a role in pathologic mechanisms of Graves’ ophthalmopathy by the induction of differentiation of orbital fibroblasts into adipocytes. J Korean Ophthalmol Soc 2011;52(3):338-344

키워드

Adipocyte differentiation, Gangliosides mixture, Graves’ ophthalmopathy, Orbital fibroblast