마이토마이신씨에 의해 유도된 인간 테논낭 섬유아세포의 자가포식현상

• 영문명: Autophagy of Human Tenon’s Capsule Fibroblasts Induced by Mitomycin-C
• 발행기관: 대한안과학회
• 저자명: 박찬희 박래길 유성광 이동욱,Channy Park, PhD,, Raekil Park, MD, PhD,, Seong Kwang Ryu, MD, Dong Wook Lee, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume52,number11, 1337~1343쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2011.11.15

영문 초록

Purpose: The present study investigated whether an autophagic process is involved in the apoptotic death of human tenon’s capsule fibroblasts (HTCFs) caused by mitomycin-C. Methods: An autophagic phenotype was tested using fluorescence microscopy and flow cytometry with specific biological staining dyes including monodansylcadaverine and acridine orange and microtubule-associated protein 1 light chain 3 (LC3). Results: Treatment with mitomycin-C (0.4 mg/ml) increased the acidic vesicular organelles of tenon’s capsule fibroblasts in a time dependent manner. Mitomycin-C induced both LC3-II cleavage and beclin-1 expression. 3-MA, a pharmacological inhibitor of autophagy, inhibited the mitomycin-C induced increase of acidic vesicular organelleS. Conclusions: Autophagy was induced with 0.4 mg/ml mitomycin-C in tenon’s capsule fibroblasts. And, autophagic mechanisms may be involved in the early stage of apoptosis of fibroblasts. J Korean Ophthalmol Soc 2011;52(11):1337-1343

키워드

Autophagy, Fibroblast, Mitomycin-C