겔형태 인공누액이 각막실질세포 및 결막상피세포에 미치는 영향

• 영문명: Effects of Gel Type Artificial Tears on Human Corneal Keratocytes and Conjunctival Epithelial Cells
• 발행기관: 대한안과학회
• 저자명: 이종수 김은희 김수진 이지은 김나미,Jong Soo Lee, MD, PhD, Eun Hee Kim, MD, Soo Jin Kim, MD, Ji Eun Lee, MD, PhD, Na Mi Kim, MD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume51,number12, 1643~1651쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2010.12.15

영문 초록

Purpose: To evaluate the biological effects and cytotoxicity of gel-type artificial tears on human corneal keratocytes and conjunctival cells in vitro. Methods: Human corneal keratocytes and conjunctival epithelial cells were exposed to Soothe® and Systane® at variable concentrations. Evaluations were conducted through an MTT-based calorimetric assay to measure the metabolic activity and through a lactate dehydrogenase (LDH) assay to assess cellular damage. Apoptotic response was examined using fluorescent microscopy and flow cytometric analysis, and cellular morphologic results were evaluated with a transmission electron microscope. Results: The inhibitory effects of corneal keratocyte and conjunctival cell proliferations increased at higher concentrations and longer exposure times to Soothe® and Systane®. The LDH titers increased after Soothe® exposure, but showed no significant difference after Systane® exposure. Soothe® and Systane® treatments both produced fluorescence, representing apoptotic cells. In flow cytometry, the maximal apoptotic response was observed for both types of artificial tears, although Systane® showed less edema, as well as reduced cytoplasmic and nuclear cell degeneration compared to those of Soothe®. Conclusions: The apoptotic responses of Soothe® and Systane® are associated with inhibitory effects of human corneal keratocyte and conjunctival epithelial cell proliferations. To inhibit the cellular proliferation of human corneal keratocytes and conjunctival epithelial cells, Systane® may be less severe than Soothe® at higher concentrations and longer exposure times. J Korean Ophthalmol Soc 2010;51(12):1643-1651

키워드

Apoptosis, Human conjunctival epithelial cell, Human corneal keratocytes, Soothe® Systane®