트리암시놀론의 망막색소상피세포 증식과 이동성 효과에 대한 일산화질소의 역할

• 영문명: Role of Nitric Oxide in the Proliferative and Migratory Effect of Triamcinolone in RPE Cells
• 발행기관: 대한안과학회
• 저자명: 김재우 이재형 이승희,Jae Woo Kim, MD, PhD, Jae Hyung Lee, MD, Seung Hee Lee, MD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume51,number1, 120~125쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2010.01.15

영문 초록

Purpose: To investigate the role of nitric oxide (NO) on the proliferative and migratory effects of triamcinolone acetonide (TA) in retinal pigment epithelial cells. Methods: After exposure to 10 nM, 1 μM, or 100 μM TA for four days, with or without co-exposure of antioxidant N-acetylcyteine, the proliferation and nitrite production of ARPE19 cells were assessed with MTT and Griess assays, respectively. Additionally, a cell migration assay was performed. Results: Cellular survival increased after exposure to TA at low concentration but decreased at high concentration. TA decreased the production of NO and cellular migration significantly, and these effects were abolished by N-acetylcysteine. Conclusions: TA showed a biphasic response on the proliferation and decreased cellular migration in ARPE19 cells, which may be mediated by nitric oxide. J Korean Ophthalmol Soc 2010;51(1):120-125

키워드

ARPE19, Migration, Nitric oxide, Triamcinolone acetonide