당뇨쥐 OLETF에서 망막조직의 미세구조적 변화

• 영문명: Ultrastructural Investigation of the Retinal Changes in Diabetic Rat (OLETF)
• 발행기관: 대한안과학회
• 저자명: 박준모 이지은 엄부섭,Jun Mo Park, MD, Ji Eun Lee, MD, PhD, Boo Sup Oum, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume51,number1, 112~119쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2010.01.15

영문 초록

Purpose: To compare retinal ultra-structures of diabetic rats (OLETF, Otsuka Long-Evans Tokushima Fatty) with those of agematched non-diabetic rats (LETO, Long-Evans Tokushima Otsuka) using transmission electron micrography (TEM). Methods: The body weights and blood sugar levels of the OLETF rats and LETO rats (n=5) were measured at 10 and 50 weeks of age. Using a TEM, we compared the ultra-structural changes between the retinas of the 50-week-old OLETF and LETO rats. We analyzed the sizes of the pericytes and the thicknesses of the retinal capillary basement membranes between the two groups. Comparisons were made using a Scion ImageⓇ. Results: The mean body weight and blood sugar levels of the 50-week-old OLETF rats were significantly higher than those of the LETO rats (p<0.05). The thicknesses of the retinal capillary basement membranes in the outer plexiform layer and the size of pericytes were significantly increased in the OLETF rats at 50 weeks of age (p<0.05). The number of nuclei in the inner nuclear layer and the outer nuclear layer (photoreceptor cell nuclei) significantly decreased (p<0.05). However, the height of the RPE cells and basal in-foldings showed no significant differences between the OLETF and LETO rats. Conclusions: The retinal changes in the OLETF rats were observed relatively early at 50 weeks of age. These changes are similar to those seen in human diabetic retinopathy. Change in the capillaries is one feature of early retinal change. OLETF rats may be a useful animal model in NIDDM to examine diabetic retinal changes. J Korean Ophthalmol Soc 2010;51(1):112-119

키워드

Diabetic retinopathy, Electron microscopy, OLETF rat, Scion ImageⓇ Spontaneously diabetic animal model