손상 받은 각막 상피세포가 중배엽 줄기세포의 분화에 미치는 영향

• 영문명: Effects of Damaged Human Corneal Epithelial Cells on Differentiation of Human Mesenchymal Stem Cell
• 발행기관: 대한안과학회
• 저자명: 신미선 홍현숙 손영숙 김재찬,Mi Sun Shin, M.S., Hyun Sook Hong, M.S., Young Sook Son, Ph.D., Jae Chan Kim, M.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume48,number3, 423~430쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2007.03.31

영문 초록

Purpose: To identify the effects of microenvironmental changes caused by human corneal epithelial damages to characteristics or differentiation of human mesenchymal stem cells (hMSCs). Methods: Artificial corneal damage was induced onto a cultured monolayer of human corneal epithelial cells. hMSCs were then co-cultured with damaged human corneal epithelial cells (dIHCE). Morphological changes in the co-cultured hMSCs were observed. To elucidate the differentiation of hMSCs into corneal keratocytes or epithelial cells, the expressions of α-smooth muscle actin, keratin-3/-12, and E-cadherin were confirmed by immunofluorescence. Results: hMSCs co-cultured with dIHCE showed enhanced adherence in the neighborhood of dIHCE and morphological change into dendritic shapes at 6 days post-seeding. Although the expression of α-smooth muscle actin, known as hMSCs marker, significantly decreased at the dIHCE-contacted site of hMSCs; there were no expressional changes on keratin-3/-12 and E-cadherin, the markers of corneal epithelial cells. Interestingly, positive expression of corneal epithelial marker keratin-3/-12 was observed in dIHCE co-cultured hMSCs. hMSCs co-cultured with normal human corneal epithelial cells (nIHCE) were unable to attach, and showed no change in the expression of α-smooth muscle actin. Conclusions: It is proposed that dIHCE causes a morphological change in hMSCs, and decreased expression of α-smooth muscle actin. These results suggest that dIHCE can affect a change in the characteristics and differentiation of hMSCs.

키워드

Mesenchymal stem cell, Cornea epithelial cell, Coculture, α-smooth muscle actin