양막추출물이 테논낭 섬유아세포에 미치는 영향

• 영문명: Effect of Amniotic Membrane Extract on the Tenon’s Capsule Fibroblasts
• 발행기관: 대한안과학회
• 저자명: 허현 김재우,Hyun Heo, M.D., Jae Woo Kim, M.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume47,number10, 1662~1668쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2006.10.31

영문 초록

Purpose: To investigate the effect of amniotic membrane extract (AME) on the survival, nitric oxide production by cultured human Tenon`s capsule fibroblasts (HTCF) and contraction of collagen gels. Methods: After exposure to 5～20 ?g/ml AME for 5 days, the survival and nitrite production of the primarily cultured HTCF were assessed with MTT and Griess assays respectively. Acridine orange/Hoechst 33342 double staining and flow cytometric analysis using FITC-Annexin/propidium iodide double staining were done to evaluate AME-induced apoptosis of HTCF. The effect of AME on the contraction of collagen gels cultured with fibroblast was also evaluated. Results: AME decreased cellular survival in a dose-dependent manner and did not affect the production of nitric oxide. AME caused apoptotic death of HTCF in a dose-dependent manner and caused necrosis at high concentration (20 ?g/ml). AME did not affect on the contraction of collagen gel. Conclusions: AME decreased cellular survival of HTCF by apoptosis and did not affect the contraction of collagen gels. Thus AME could modulate conjunctival wound healing by suppressing the cellular survival and activity of HTCF.

키워드

Amniotic membrane extract, Apoptosis, Contraction, Fibroblast, Wound healing