Regeneration of somaclones from explants of internodal stems of Mentha piperita and M. spicata

• 발행기관: 강원대학교 기초과학연구소
• 저자명: Joon Chul Kim
• 간행물 정보: 『기초과학연구』제24집, 43~51쪽, 전체 8쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 2013.12.31

영문 초록

Somaclones were regenerated from explants of internodal stems of Murray Micham and Black Mitcham peppermint (Mentha piperita) and Scotch spearmint (M. spicata) on Murashige-Skoog (MS) basal medium combinated with thidiazuron, 6-benzylaminopurine, indole-3-acetic acid and indole-3-butyric acid. The combination best suited for regeneration was MS medium supplemented with 2 mg indole-3-acetic acid and 2 mg thidiazuron per liter of medium. On this regenerative medium, nearly all explants of the peppermint cultivars regenerated numerous somaclones, but less than 20% of exlplants of Scotch spearmint produced somaclones. Depending on the medium used, somaclones were regenerated from explants of Murray Mitcham peppermint within 2 to 5 weeks, from Black Mitcham peppermint within 3-5 weeks, and from Scotch spearmint in 4-10 weeks. Explants of Native spearmint (Mentha spicata) produced only roots on the medium with 2 mg/L NAA and 1 mg/L BAP.

목차

Abstract

1. Introduction

2. Materials and Methods

3. Results

4. Discussion

5. References

키워드

Tissue culture somaclones peppermint spearmint