방사선조사를 받은 흰쥐 뇌실 맥락얼기의 미세구조변화

• 영문명: Ultrastructural Changes of the Ventricular Choroid Plexus of the Rat following Irradiation on the Head
• 발행기관: 대한해부학회
• 저자명: 고정식(Jeong-Sik Ko) 최숙경(Sook-Kyung Choi) 안의태(E-Tay Ahn) 박경호(Kyung-Ho Park) 김진국(Jin-Gook Kim)
• 간행물 정보: 『대한해부학회지』제31권 제6호, 847~859쪽, 전체 13쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1998.12.30

국문 초록

과량의 방사선조사가 맥락얼기에 미치는 영향을 알아보기 위해 이 실험을 시행하였다. 체중 200~250 g의 숫흰쥐의 머리 부분에 3,000 rad 또는 6,000 rad선량의 방사선을 조사한 후 6시간, 2일, 6일 후에 동물을 희생시켰고, 통상적인 전자현미경표본제작법에 따라 조직절편을 만든 다음 전자현미경으로 관찰하여 다음과 같은 결론을 얻었다. 1. 3,000 rad 조사군에서는 맥락상피세포의 미세융모와 바닥안주름의 소실, 자유모서리와 바닥면 세포막에서의 세포막함입소포 및 운반소포의 감소, 골지복합체의 위축 등, 물질이동기구가 위축되었다. 위축된 정도는 6시간에 가장 뚜렷하였고, 2일에는 약간 회복되는 형태학적 소견을 보였으나, 6일에는 다시 위축되었다. 2. 6,000 rad 조사군에서는 물질이동기구의 변화가 3,000 rad 조사군에 비하여 심하였다. 3. 방사선조사군에서 모세혈관주위공간의 맥락조직세포들은 뚜렷한 형태학적 변화를 나타내지 않았다. 4. 방사선조사군에서 맥락상피세포사이의 폐쇄띠는 형태학적 변화를 보이지 않았다. 이와 같은 결과로 보아 과량의 방사선조사를 받으면 맥락얼기상피의 손상으로 뇌척수액의 분비가 급격히 감소되고, 뇌척 수액을 통한 항상성 유지에 이상이 초래됨으로써 신체기능이 크게 떨어질 것으로 생각된다.

영문 초록

Acute irradiation effects on the choroid plexus were studied. Healthy male rats (Sprague Dawley) were anesthetized with sodium thiopental, and were placed on the table of Mistubishi linear accelerator ML-4MV. The rats were irradiated on their heads and necks with the doses of 3,000 rads or 6,000 rads. Six hours, 2 days and 6 days following the irradiations, rats were sacrificed by perfusion fixation through the heart, with 1% glutaraldehyde-1% paraformaldehyde solution. Choroid plexuses of lateral ventricles were taken out and fixed in the same fixative overnight, and were postfixed in 2% osmium tetroxide solution. Fixed tissue blocks were dehydrated within series of alcohol and acetone, and were embedded in araldite mixture. Ultrathin sections were cut, and contrasted with uranyl acetate and lead citrate solutions, and observed with electron microscope. The results obstained were as follows : 1. Choroid epithelial cells of irradiated rats exhibited severe alterations of their organelles concerning intracellular material transport; such as disappearance of microvilli and basal infoldings, reduction of invaginating pits on the basal and apical plasma membranes, reduction of transfer vesicles, shrinked Golgi complexes, etc. 2. In choroidal epithelial cells of the rats exposed with doses of 6,000 rads, the morphological alterations of the intracellular transport system were more severe than those of the rats exposed with doses of 3,000 rads. 3. Cellular elements and collagen fibrils within the pericapillary space were not so much affected following irradiations. 4. Zonula occludentes in between choroidal epithelials cells were not altered after the irradiations. The above results indicate that the production of cerebrospinal fluid by choroid plexus is severely reduced. Since the choroid plexus functions not only the secretions but it also concerns the homeostasis of many important ions, neuromodulaters, and other peptides, heavy irradiation on the head may result severe malfunction of central nervous system and humoral homeostasis.

키워드

방사선조사 맥락얼기 전자현미경 흰쥐 Radiation Choroid plexus Electron microscopy Rat