Somatic embryogenesis and plant regeneration from immature zygotic embryos of Japanese larch(Larix leptolepis)

• 영문명: Somatic embryogenesis and plant regeneration from immature zygotic embryos of Japanese larch(Larix leptolepis)
• 발행기관: 강원대학교 기초과학연구소
• 저자명: Yong Wook Kim Yang Youn Eu Rae Noh Joon Chul Kim
• 간행물 정보: 『기초과학연구』제10집, 183~189쪽, 전체 7쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 1999.12.01

영문 초록

Embryogenesis tissue was initiated using LM, LP and MS media from open-pollinated immature embryos of Larix leptolepis. The initiation frequency varied with collection dates. The highest frequencies of embryogenic tissue initiation (60,67 and 59% on LM, LP and MS media, respectively) were observed from cones collected on July 30. At this time, all the excised embryos were at the cotyledonary stage. ABA over a wide concentration and length of exposure range did not promote maturation, but was beneficial in reducing precocious germination. Of over 400 plants regenerated, 72 were transplanted into soil mixtures and to date, 69 of these (95%) have survived.

목차

Introduction

Materials and method

Results and discussion

References