The Response of Several Enzyme Activities from Arabidopsis thaliana after the Treatment with Mercury

• 영문명: The Response of Several Enzyme Activities from Arabidopsis thaliana after the Treatment with Mercury
• 발행기관: 강원대학교 기초과학연구소
• 저자명: Keum-Nam Park Chang-Jin Lim
• 간행물 정보: 『기초과학연구』제10집, 17~24쪽, 전체 8쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 1999.12.01

영문 초록

Three-week grown Arabidopsis thaliana leaves were wounded by cutting whole leaves with a razor blade into pieces (about 3 mm × 3 mm), submerged in solutions containing various concentrations of mercuric chloride (0, 0.05, 0.1 and 0.2 mM), and incubated in a growth chamber for 24 h at 25°C. We measured and compared activities of various enzymes such as phenylalanine ammonia-lyase, tyrosine ammonia-lyase, thioredoxin, thioltransferase, and glutathione reductase. Treatments leaves with mercury resulted in a dose-dependent decrease in activities in phenylalanine ammonia-lyase, thioredoxin, and thioltransferase. Whereas glutathione reductase activity was drastically decreased at 0.05 mM mercury treatment compared to control, and then was slightly increased at concentrations more than 0.05 mM. Our results strongly indicate that sulfhydryl-related and phenylpropanoid-synthesizing enzyme activities are affected by treatment with mercuric chloride. Further studies, however, remain to elucidate the mechanisms involved.

목차

1. Introduction

2. Materials and Methods

3. Results and Discussion

References