Purification and Properties of Thioltransferase from Leaf Mustard

• 영문명: Purification and Properties of Thioltransferase from Leaf Mustard
• 발행기관: 강원대학교 기초과학연구소
• 저자명: Jae-Hoon Sa Kyung-Min Kim Eun-Hee Kim Chang-Jin Kim
• 간행물 정보: 『기초과학연구』제8집, 28~38쪽, 전체 11쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 1997.12.01

영문 초록

Thioltransferase, which is required for glutathione (GSH)-dependent formation of deoxyribonucleotides by ribonucleotide reductase, has been identified and purified to homogeneity from leaf mustard, The isolation procedure involved four chromatography steps on DEAE-cellulose, Sephadex G-l00 (2nd), and Q-Sepharose, The final preparation was homogenous as judged by polyacrylamide gel electrophoresis, Its molecular weight was estimated to be about 15 kDa, The purified enzyme was characterized on several aspects such as optimal pH, Km, and Vmax

목차

Abstract

Introduction

Materials and Methods

Results and Discussion

References