Isolation, Characterization, and Comparative Genomics of the Novel Potential Probiotics from Canine Feces

• 영문명: Isolation, Characterization, and Comparative Genomics of the Novel Potential Probiotics from Canine Feces
• 발행기관: 한국축산식품학회
• 저자명: Ngamlak Foongsawat Sirinthorn Sunthornthummas Kwannan Nantavisai Komwit Surachat Achariya Rangsiruji
• 간행물 정보: 『Food Science of Animal Resources』Food Science of Animal Resources 제43권 제4호, 685~702쪽, 전체 18쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2023.07.31

영문 초록

Lactic acid bacteria (LAB) are commonly used as probiotics; however, not all LAB strains have the same beneficial effects. To successfully use LAB as probiotics in canines, LAB species should originate from the canine intestinal tract as they display host specificity. The objective of this study was to investigate the phenotypic and genomic traits of potential probiotic LAB isolated from canine fecal samples. Twenty LAB samples were evaluated for their potential probiotic characteristics including resistance to low pH, bile salts, hydrophobicity, auto-aggregation, co-aggregation, adhesion to epithelia or mucosa, and production of inhibitory compounds. Additionally, we evaluated their safety and other beneficial effects on canine health, such as DPPH free radical scavenging, and β-galactosidase. Four strains demonstrated potential probiotic characteristics and were selected: Enterococcus hirae Pom4, Limosilactobacillus fermentum Pom5, Pediococcus pentosaceus Chi8, and Ligilactobacillus animalis FB2. Safety evaluations showed that all strains lacked hemolytic activity, could not produce biogenic amines, and did not carry any pathogenic genes. In addition, L. fermentum Pom5 and P. pentosaceus Chi8 displayed susceptibility to all antibiotics and concordant with the absence of antibiotic resistance genes. Based on their phenotypic and genomic characteristics, L. fermentum Pom5 and P. pentosaceus Chi8 were identified as potential probiotic candidates for canines.

목차

Introduction

Materials and Methods

Results and Discussion

Conclusion

Supplementary Materials

Conflicts of Interest

Acknowledgements

Author Contributions

Ethics Approval

References

키워드

Limosilactobacillus fermentum Pediococcus pentosaceus lactic acid bacteria probiotics canine