ITS 영역의 HRM 분석을 통한 참당귀(Angelica gigas Nakai)의 특이적 SNP 분자표지 개발

• 영문명: Development of specific single nucleotide polymorphism molecular markers for Angelica gigas Nakai
• 발행기관: (사)한국식물생명공학회
• 저자명: 이신우(Shin-Woo Lee) 이수진(Soo Jin Lee) 한은희(Eun-Hee Han) 신용욱(Yong-Wook Shin) 김윤희(Yun-Hee Kim)
• 간행물 정보: 『Journal of Plant Biotechnology』48권 2호, 71~76쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2021.06.30

영문 초록

Angelica is a perennial plant used widely for medicinal purposes. Information on the genetic diversity of Angelica populations is important for their conservation and germplasm utilization. Although Angelica is an important medicinal plant genus registered in South Korea, no molecular markers are currently available to distinguish individual species from other similar species in different countries, in particular, China and Japan. In this study, we developed single nucleotide polymorphism (SNP) markers derived from internal transcribed spacer regions of the nuclear ribosomal DNA to identify a distinct domestic species, Angelica gigas Nakai, via a high-resolution melting (HRM) curve analyses. We also performed HRM curve analysis of intentionally mixed genomic DNA samples from five Angelica species. Finally, we investigated A. gigas Nakai and A. sinensis using varying ratios of mixed genomic DNA templates. The SNP markers developed in this study are useful for rapidly identifying A. gigas species from different countries.

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키워드

Angelica High-resolution melting Internal transcribed spacer regions of rDNA Single nucleotide polymorphisms