Initiation, proliferation, and improvement of a micropropagation system for mass clonal production of banana through shoot-tip culture

• 발행기관: (사)한국식물생명공학회
• 저자명: Allah Jurio Khaskheli Muharam Ali Syad Zakir Hussain Shah Zohra Fatima Memon Saleem Awan Muhammad Ib
• 간행물 정보: 『Journal of Plant Biotechnology』48권 2호, 86~92쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2021.06.30

영문 초록

The present study aimed to evaluate the initiation, proliferation potential, and mass clonal production ability of a micropropagation system for banana through tissue culture. A total of 60 explants were cultured on basal media supplemented with various concentrations of BAP and NAA. Banana plants regenerated on MS basal medium (control) without the addition of BAP + NAA showed a significantly (P < 0.05) lower survival rate with no signs of shoots up to the end of the experimental period. The results further revealed that the performance in MSS-XI medium was almost 89%, followed by MSS-IX and MSS-X media, both of which showed performance up to 88%. In contrast, the performance in the MSS-XVI medium was less than 60%, at the less duration of time and highly shoot induction detected at MSS-XIII medium. The maximum number of shoots (4.9) was observed in the medium supplemented with growth adjuster MSS-XI, followed by the MSS-XII medium (4.5). Surprisingly, the best performance was observed for the MSR-VII medium approximately 16 days after initiation, while the lowest performance was observed with MSR-XI (approximately 31 days). The maximum rooting percentage (98%) was observed in the MSR-V to MSR-VIII media (98%), while the minimum rooting percentage was observed in MSR-XI (approximately 45%)

목차

Introduction

Materials and Methods

Results and Discussion

Conclusions

Conflict of Interest Disclosures

Authors’ Contributions

Acknowledgement

References

키워드

Banana Tissue culture Hormones Regeneration Shoots Roots