성숙한 3T3-L1 지방세포에서 Akkermansia muciniphila의 지방축적 감소 효과

• 영문명: Effect of Reduction in the Adipose Accumulation of Akkermansia muciniphila in Mature 3T3-L1 Adipocytes
• 발행기관: 한방비만학회
• 저자명: 심혜윤(Hyeyoon Shim) 임수경(Sookyoung Lim) 신주현(Joo-Hyun Shin) 이도경(Dokyung Lee) 서재구(Jae-Gu Seo) 김호준(Hojun K
• 간행물 정보: 『대한한방비만학회지』제19권 2호, 106~112쪽, 전체 7쪽
• 주제분류: 의약학 > 한의학
• 파일형태: PDF
• 발행일자: 2019.12.31

영문 초록

Objectives: The aim of this study was to observe the reduction of lipid accumulation by treatment with Akkermansia muciniphila extract on 3T3-L1 adipocytes. Methods: After treating pasteurized Akk. muciniphila strains in HT-29 colorectal cancer cell, the relative expression of interleukin (IL)-8, tumor necrosis factor-α, IL-6, and IL-1β mRNA was analyzed by real time polymerase chain reaction, respectively. 27 strains of Akk. muciniphila which have anti-inflammatory effects were selected. 3T3-L1 pre-adipocytes were treated with Akk. muciniphila for 24 hr and then measured the toxicity using water soluble tetrazolium salt assay. The cells were incubated for 4 days and then differentiated into adipocytes using the medium including adipogenic reagents for 10 days. The Akk. muciniphila was treated when the medium was exchanged for differentiation medium at 4th day and insulin medium at 6th day. To observe the lipid accumulation, the cells were stained with Oil red O dye and were measured using a spectrophotometer. Results: In the cytotoxicity test, the cell viability of 3T3-L1 pre-adipocytes was significantly increased compared to the control group which untreated with Akk. muciniphila, and there was no cytotoxicity of Akk. muciniphila at 1×107 CFU/mL. The results on Oil red O staining and absorbance measurements were showed a significant decrease in lipid accumulation in the group which was treated with Akk. muciniphila compared to the control group. Conclusions: In our results, Akk. muciniphila has the inhibitory effect of lipid accumulation in 3T3-L1 adipocytes. This suggests that Akk. muciniphila could be help to improve obesity.

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키워드

Akkermansia muciniphila Adipocytes Microbiota Obesity